The promoter of filamentation (POF1) protein from Saccharomyces cerevisiae is an ATPase involved in the protein quality control process
Costa, Iris M; Nasser, Tallybia H. T; Demasi, Marilene; Nascimento, Rafaella M. P; Netto, Luis E. S; Miyamoto, Sayuri; Prado, Fernanda M; Monteiro, Gisele.
BMC microbiol
; 11(268): 1-11, Dec 28, 2011.
Artículo
en Inglés
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO | ID: biblio-1060416
The gene YCL047C, which has been renamed promoter of filamentation gene (POF1), has recentlybeen described as a cell component involved in yeast filamentous growth. The objective of this work is tounderstand the molecular and biological function of this gene.Here, we report that the protein encoded by the POF1 gene, Pof1p, is an ATPase that may be part of theSaccharomyces cerevisiae protein quality control pathway. According to the results, Äpof1 cells showed increasedsensitivity to hydrogen peroxide, tert-butyl hydroperoxide, heat shock and protein unfolding agents, such asdithiothreitol and tunicamycin. Besides, the overexpression of POF1 suppressed the sensitivity of Äpct1, a strain thatlacks a gene that encodes a phosphocholine cytidylyltransferase, to heat shock. In vitro analysis showed, however,that the purified Pof1p enzyme had no cytidylyltransferase activity but does have ATPase activity, with catalyticefficiency comparable to other ATPases involved in endoplasmic reticulum-associated degradation of proteins(ERAD). Supporting these findings, co-immunoprecipitation experiments showed a physical interaction betweenPof1p and Ubc7p (an ubiquitin conjugating enzyme) in vivo.Taken together, the results strongly suggest that the biological function of Pof1p is related to the regulation of protein degradation.
Biblioteca responsable:
BR78.1
Ubicación: BR78.1
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