Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line
Laboratório de Biologia Molecular e Doenças EndêmicasCôrtes, Luzia Monteiro de Castro; Laboratório de Biologia Molecular e Doenças Endêmicasde Pita-Pereira, Daniela; Laboratório de Biologia Molecular e Doenças EndêmicasFarani, Priscila Silva Grijó; Laboratório de Biologia Molecular e Doenças EndêmicasPereira, Bernardo Acácio Santini; Laboratório de Biologia Molecular e Doenças EndêmicasDias-Lopes, Geovane; Laboratório de Biologia Molecular e Doenças Endêmicasda Silva, Franklin Souza; Laboratório de Genômica Funcional e BioinformáticaCorrêa, Paloma Resende; Plataforma de Microscopia Eletrônica Rudolf BarthSilva, Roger Magno Macedo; Laboratório de Biologia EstruturalCôrte-Real, Suzana; Bello, Felio Jesus; Laboratório de Genômica Funcional e BioinformáticaMendonça-Lima, Leila; Laboratório de Biologia Molecular e Doenças EndêmicasMoreira, Otacilio da Cruz; Laboratório de Genômica Funcional e BioinformáticaWaghabi, Mariana Caldas; Laboratório de Biologia Molecular e Doenças EndêmicasAlves, Carlos Roberto.
Mem. Inst. Oswaldo Cruz
; 115: e200113, 2020. tab, graf
Artículo
en Inglés
| LILACS, SES-SP | ID: biblio-1135235
BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. METHODS Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase. RESULTS Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). CONCLUSIONS This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.
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