Abstract Midgut transgenic
bacteria can be used to express and deliver anti-
parasite molecules in
malaria vector
mosquitoes to reduce
transmission. Hence, it is necessary to know the symbiotic
bacteria of the
microbiota of the midgut to identify those that can be used to interfering in the vector
competence of a target
mosquito population. The bacterial
communities associated with the
abdomen of Nyssorhynchus braziliensis (Chagas) (
Diptera Culicidae) and Nyssorhynchus darlingi (Root) (
Diptera Culicidae) were identified using Illumina NGS sequencing of the V4 region of the
16S rRNA gene. Wild
females were collected in rural and periurban
communities in the Brazilian
Amazon.
Proteobacteria was the most abundant group identified in both species. Asaia (
Rhodospirillales Acetobacteraceae) and
Serratia (Enterobacterales Yersiniaceae) were detected in Ny. braziliensis for the first
time and its presence was confirmed in Ny. darlingi.