Further insights into the eco-epidemiology of American cutaneous leishmaniasis in the Belem metropolitan region, Pará State, Brazil
Gonçalves, Lucas Pantoja; Santos, Thiago Vasconcelos dos; Campos, Marliane Batista; Lima, Luciana Vieira do Rêgo; Ishikawa, Edna Aoba Yassui; Silveira, Fernando Tobias; Ramos, Patrícia Karla Santos.
Rev. Soc. Bras. Med. Trop
; 53: e20200255, 2020. tab, graf
Artículo
en Inglés
| SES-SP, Coleciona SUS (Brasil), LILACS | ID: biblio-1143862
Abstract
INTRODUCTION:
In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RNAPOIILS)-polymerase chain reaction (PCR) followed by analysis of restriction fragment length polymorphism (PCR-RFLP) to identify Leishmania spp. ACL agents in this region.METHODS:
Thirty-two Leishmania spp. isolates from patients with ACL in the BMR during 1995-2018 were analyzed. Leishmania spp. DNA samples were amplified using the primers RPOR2/RPOF2, and the 615-bp PCR products were subjected to enzymatic digestion using TspRI and HgaI endonucleases.RESULTS:
ACL etiological agents in the BMR comprised Leishmania (Viannia) lindenbergi (43.7%) followed by Leishmania (Viannia) lainsoni (34.4%), Leishmania (Leishmania) amazonensis (12.5%), and Leishmania (Viannia) braziliensis (9.4%).CONCLUSIONS:
To our knowledge, the results of the study revealed for the first time that L. (V.) lindenbergi and L. (V.) lainsoni are the main ACL agents in BMR.
Biblioteca responsable:
BR1.1
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