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A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina / A species-specific method for detecting pathogenic Streptomyces species from soil and potato tubers in Argentina.
Barrera Viviana A
;
Kageyama Koji
;
Rojo Rodrigo A
;
Gasoni Laura
;
Kobayashi Kiroku
.
Rev. argent. microbiol
; 45(4): 277-81, dic. 2013.
Artículo en Español | LILACS, BINACIS | ID: biblio-1171793
Potato
common scab is caused by several
soil
-inhabiting pathogenic
Streptomyces
species. In the present study, a species-specific
PCR
method
was used to detect
Streptomyces
species in
potato
tuber lesions and
soils
. Total genomic
DNA
from
soil
samples from six locations and tuber samples from four
potato
cultivars (Spunta, Shepody, Innovator and Russet Burbank) were assessed.
Streptomyces
scabies
,
Streptomyces
acidiscabies, and
Streptomyces
turgidiscabies were detected in
soybean
, tobacco and
potato
soils
and in all
potato
varieties except Russet Burbank. The
phylogenetic analysis
of the sequences obtained confirmed the identification. The
method
proposed proved to be
time
-saving and
cost
effective for the rapid
detection
of
Streptomyces
species. This is the first
report
of the
detection
of S. acidiscabies and S. turgidiscabies in
soils
and
potato
tubers from
Argentina
.
Asunto(s)
Enfermedades de las Plantas/microbiología
Microbiología del Suelo
Solanum tuberosum/microbiología
Streptomyces/aislamiento & purificación
Argentina
Especificidad de la Especie
Técnicas Bacteriológicas/métodos
Biblioteca responsable:
AR5.1
Ubicación: [{"text": "AR5.1"}]
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