The PolioLaboratory Network has always played a critical role in diagnosing poliovirusdisease (poliomyelitis) and the detection of poliovirustransmission. In the new millennium; the strength of the laboratory network is often a direct reflection of the success of the Polio Eradication Initiative (PEI) programme. The network has taken advantage of new technologies that provide speedy turnaround times for results reporting thus contributing to the success of the PEI programme. This article presents a brief overview of the work of the network