Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display / Seleção e caracterização de peptídeos miméticos a Campylobacter fetus subsp. venerealis usando phage display
Ribeiro, Eduardo Lara; Fujimura, Patrícia Tiemi; Ueira-Vieira, Carlos; Goulart, Luiz Ricardo; Alves, Telma Maria; Lage, Andrey Pereira; Heinemann, Marcos Bryan.
Ciênc. rural (Online)
; 51(8): e20200945, 2021. tab, graf
Artículo
en Inglés
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LILACS-Express
| ID: biblio-1278898
ABSTRACT Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.
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