Mozambique introduced the rotarix®
vaccine (gsk
biologicals , rixensart,
belgium ) into the na-tional
immunization program in september 2015. Although g1p[8] was one of the most prevalent
genotypes between 2012 and 2017 in
mozambique , no complete
genomes had been sequenced to date. here we
report whole
genome sequence analysis for 36 g1p[8]
strains using an illumina miseq platform. all
strains exhibited a wa-like genetic backbone (g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1).
phylogenetic analysis showed that most of the mozambican
strains clustered closely to-gether in a conserved clade for the entire
genome . no distinct
clustering for pre- and post-
vaccine strains were observed. these findings may suggest no selective
pressure by the introduction of the rotarix®
vaccine in 2015. two
strains (hjm1646 and hgm0544) showed varied
clustering for the entire
genome , suggesting reassortment, whereas a further
strain obtained from a
rural area (man0033) clustered separately for all
gene segments.
bayesian analysis for the vp7 and vp4 en-
coding gene segments supported the
phylogenetic analysis and indicated a possible introduction from
india around 2011.7 and 2013.0 for the main mozambican clade. continued
monitoring of
rotavirus strains in the post-
vaccine period is required to fully understand the impact of
vaccine introduction on the diversity and evolution of
rotavirus strains .