FOXP3 variants are independently associated with transforming growth factor B1 plasma levels in female patients with inflammatory bowel disease
Inoue, Cláudia Junko; Flauzino, Tamires; Goncalves, Beatriz Piantoni; Paula, Jaqueline Costa Castardo de; Galvão, Talita Cristina; Miyazaki, Paula Kikuchi; Alcantara, Camila Cataldi de; Westmore, Lucilene Rosa e Silva; Lozovoy, Marcell Alysson Batisti; Reiche, Edna Maria Vissoci; Simão, Andréa Name Colado.
Clinics
; 77: 100084, 2022. tab, graf
Artículo
en Inglés
|
LILACS-Express
| ID: biblio-1404304
Abstract
Objective:
The aim of this study was to evaluate the association of -924 G>A (rs2232365) and -3279 C>A (rs3761548) FOXP3 variants with IBD susceptibility, clinical and endoscopic activity, and IL-10 and TGF-β1 plasma levels.Method:
The study included 110 IBD female patients, 60 with Ulcerative Colitis (UC) and 50 with Crohn's Disease (CD), and 154 female controls. FOXP3 variants were determined with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Plasma levels of IL-10 and TGF-β1 were determined using immuno-fluorimetric assay.Results:
AA genotype of rs2232365 and rs3761548 was associated with CD (OR = 3.147, 95% CI 1.015-9.758, p = 0.047) and UC (OR = 3.221, 95% CI 1.050-9.876, p = 0.041) susceptibility, respectively. However, were not associated with TGF-β1 and IL-10 levels, and endoscopic/clinical activity disease. GAGA haplotype was associated with IBD (OR = 4.003, 95% CI 1.100-14.56, p = 0.035) and UC susceptibility (OR = 6.107, 95% CI 1.609-23.18, p = 0.008). In addition, IBD patients with the GAGA haplotype had lower TGF-β1 levels (p = 0.041). Moreover, G/C haplotype (dominant model) had a protective effect of 60% in CD susceptibility and lower Endoscopic Severity Index.Conclusions:
These results suggest that FOXP3 variants could exert a role in the Treg, which could be one of the factors involved in the susceptibility and pathogenesis of IBD.
Biblioteca responsable:
BR1.1
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