An RT-rtPCR assay for detection of rabies virus in bovine specimens / Um ensaio RT-rtPCR para detecção de vírus da raiva em espécimes bovinos
Almeida, Gisane Lanes de; Monteiro, Francielle Liz; Merchioratto, Ingryd; Mortari, Ana Paula Gnocato; Cargnelutti, Juliana Felipetto; Weiblen, Rudi; Flores, Eduardo Furtado.
Ciênc. rural (Online)
; 53(1): 1-7, 2023. tab, graf
Artículo
en Inglés
| VETINDEX | ID: biblio-1410644
Bovine rabies is endemic in most Brazilian States, including Rio Grande do Sul (RS), which has faced an unprecedented rabies outbreak between 2011 and 2018. We described a real-time reverse transcription quantitative PCR (RT-rtPCR) for detection of rabies virus (RABV) in bovine samples. The primers were designed targeting a highly conserved region of the nucleoprotein (N) gene of RABV obtained from cattle. The detection limit corresponded to 13 DNA copies and the intra- and inter-run repeatability was adequate (CV<9%) in all dilutions tested. Amplification of other pathogens associated with neurological disease in cattle or cross-contamination was not observed. Brain samples from cattle suspicious of rabies (n=21) were tested in triplicate by the RT-rtPCR and by the gold-standard direct fluorescent antibody test (DFAT), resulting in 100% of sensitivity and specificity of the RT-rtPCR. Testing of additional 41 bovine brain samples submitted to the routine DFAT testing yielded 37 (90.2%) concordant results (30 positive/7 negative) and 4 (9.7%) inconclusive in DFAT and RT-rtPCR positive. These results showed a good concordance between the tests and a higher sensitivity of the RT-rtPCR. This assay represents an alternative for RABV detection, either as a confirmatory test or for large-scale diagnosis in endemic regions.
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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