BACKGROUND
Dengue is a
disease caused by
dengue virus (DENV-1 through -4). Among the four
serotypes, DENV-4 remains the least studied.
Acute kidney injury is a potential complication of
dengue generally associated with
severe dengue infection. OBJECTIVES The
goal of this study was to investigate the alterations caused by experimental
dengue infection in the
kidney of
adult BALB/c
mice.
METHODS In this study, BALB/c
mice were infected through the intravenous route with a DENV-4
strain, isolated from a
human patient. The
kidneys of the
mice were procured and subject to histopathological and ultrastructural
analysis. FINDINGS The presence of the
viral antigen was confirmed through
immunohistochemistry.
Analysis of
tissue sections revealed the presence of inflammatory
cell infiltrate throughout the parenchyma. Glomerular enlargement was a common find.
Necrosis of tubular
cells and haemorrhage were also observed.
Analysis of the
kidney on a
transmission electron microscope allowed a closer look into the necrotic tubular
cells, which presented nuclei with condensed
chromatin, and loss of
cytoplasm. MAIN CONCLUSIONS Even though the
kidney is probably not a primary target of
dengue infection in
mice, the inoculation of the
virus in the
blood appears to damage the renal
tissue through local
inflammation.