In this study, we reported the evaluation of ruminal
parameters and the rate and degree of degradation of
biogenic amines (BA)
in vitro, using ruminal fluid from
sheep fed two different
diets (
meadow hay and
silage of
Medicago sativa L.). Herein, the Charolais
sheep breed with a ruminal
cannula was used to gather samples. Multiple
rumen parameters were evaluated after 10 days of the
feeding period. Samples were processed using fermenters inoculated by BA (
histamine,
tyramine,
putrescine,
cadaverine,
spermine, and
spermidine) during 8-h
fermentation. Levels of BA were determined using
ion-exchange chromatography, and
volatile fatty acids (VFA) were determined using
gas chromatography. More rapid BA degradation was noticed in hay than in haylage. Overall, VFA levels increased with duration of the
fermentation, while
pH lowered. The rapid increase was observed after 4 h of
fermentation.
Ammonia concentration had a decreasing trend in
silage and increasing in the hay. Hypotheses on BA degradation were accepted. Important
knowledge of the development of ruminal
parameters in
time was obtained, and based on gathered information, it is possible to say that other
parameters and their impacts on BA in
rumen need to be examined further.(AU)