Molecular heterogeneity of Malassezia pachydermatis through RAPDPCR / Heterogeneidade molecular da Malassezia pachydermatis através de RAPD-PCR
Nascente, Patrícia da Silva; Coimbra, Helen Silveira; Meinerz, Ana Raquel Mano; Meireles, Mário Carlos Araújo; Mello, João Roberto Braga; Schuch, Luiz Filipe Damé.
Acta sci., Biol. sci
; 32(2): 169-173, abr.-jun.2010. ilus, tab
Artículo
en Inglés
| LILACS, VETINDEX | ID: biblio-1460647
Several methodologies in molecular biology have been used in theinvestigation of Malassezia pachydermatis and its differentiation into subtypes. Recentmolecular research of this species includes the use of samples isolated from canine otitisexterna and dermatitis, as well as from healthy animals, having in view an epidemiologicalstudy of the yeast. The aim of this study was to identify molecular differences in M.pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strainswere analyzed by means of the Random Amplification Primer DNA - Polimerase ChainReaction (RAPDPCR) for molecular heterogeneity research. DNA extraction was carriedout with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer.A variation was observed in the number and arrangement of the bands among the 49studied isolates, grouped into nine patterns. Isolate groupings were not found to be relatedto animal breed, age or sex. It was concluded that M. pachydermatis has differences in itsmolecular profile, as shown by the molecular technique (RAPD PCR), which allowsisolates to be classified into nine subtypes.
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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