Effect on supplementation of three taurine's concentration in cryopreserved goat sperm / Efeito da suplementação de três concentrações de taurina ao sêmen caprino criopreservado
Souza, Rafael Limongi de; Silva, Robespierre Augusto Joaquim Araújo; Batista, André Mariano; Guerra, Maria Madalena Pessoa; Silva, Sildivane Valcácia.
Ciênc. vet. tróp
; 18(1): 32-38, jan.-abr. 2015. tab, graf
Artículo
en Portugués
| VETINDEX | ID: biblio-1480618
This study aimed to evaluate the effect of different concentrations of taurine to post-thaw goat semen. It was used goat cryopreserved semen Boer (n=3); two pallets of each breeding animal were thawed (37oC/30 s) and samples were homogenized. Four experimental groups were formed CG= control group (semen with minimal essential medium); G1= semen + 5 mM taurine; G2= semen + 25 mM taurine; G3= semen + 50 mM taurine; assessed for sperm kinetics, plasma membrane and acrosome integrity, and mitochondrial function after the sample groups formation at thawing moment (T0), one (T1) and three (T3) hours post-thaw. Six replicates were performed. Data were subjected to variance analysis (ANOVA, One way) and Tukeys test at 5% significance level. The CG decreased (p0.05) to other kinematic parameters and integrity of sperm membranes among the treated groups and the CG. Thus, in conclusion, the taurine at concentrations 5, 25 and 50 mM preserves total motility, progressive motility, rapid sperm and beat cross frequency of goat sperm after three hours post-thaw.
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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