Background The present study compared two
methods used successfully in a large-scale program for the collection of
scorpion venoms, namely the milking of
adult scorpions via manual and
electrical stimulation. Results Our immunobiochemical characterizations clearly demonstrate that regularly applied
electrical stimulation obtains
scorpion venom more easily and, most importantly, in greater quantity. Qualitatively, the electrically collected
venom showed lack of
hemolymph contaminants such as
hemocyanin. In contrast, manual obtainment of
venom subjects
scorpions to maximal
trauma, leading to
hemocyanin secretion. Our study highlighted the importance of reducing
scorpion trauma during
venom milking. Conclusions In conclusion, to produce high quality
antivenom with specific
antibodies, it is necessary to collect
venom by the gentler
electrical stimulation method.