Fracionamento de doses de sêmen bovino criopreservado para utilização na fertilização in vitro de embriões / Fractionation of bovine semen doses cryopreserved for use in vitro fertilization
Morais, Rodrigo de; Albuquerque, Rodrigo dos Santos; Araújo, Gilson Ferreira; Gonçalves, Fábio Trindade; Sousa, Alysson Jorge de Oliveira; Silva, Aluizio Otavio Almeida da; Sousa, José Silva de; Reis, Adriana Novaes dos.
Rev. bras. reprod. anim
; 40(4): 267-269, Out-Dez. 2016. graf
Artículo
en Portugués
| VETINDEX | ID: biblio-1492260
The use of frozen semen for PIVE is not fully utilized, as usually using a full dose of semen being wasteda large number of sperm that could be used in other PIVE, for these reasons, the objective of this work was toevaluate the fractionation of 0.25 ml straws in frozen bovine semen doses, divided into four equal sections forlater use in IVF technique in order to avoid wasting sperm present in the straw. Motility, vigor and spermconcentration analyzes were performed. There was observed no effect of vane fractionation process on spermparameters evaluated, such as motility 58,75%±7,6, vigor 2,75±1,6, and spermatic concentration average(3,7x106) between the sectioned parts. Thus, it is concluded that the semen dose fractionation method ofcryopreserved bull into four sections is viable for in vitro fertilization techniques, provided that there is nocompromise on the number and viability of sperm cells.
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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