The human follicle stimulating hormone (hFSH) keeps the normal ultrastructure of caprine preantral follicles cultured in vitro / O hormônio folículo estimulante humano (hFSH) mantém a ultraestrutura normal de folículos pré-antrais caprinos cultivados in vitro
Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Benjamin, Laércio dos Anjos; Verde, Isabel Bezerra Lima; Celestino, Juliana Jales de Hollanda; Figueiredo, José Ricardo de.
Semina ciênc. agrar
; 36(3,supl.1): 1965-1978, 2015. ilus, tab, graf
Artículo
en Inglés
| VETINDEX | ID: biblio-1499992
The aim of this study was to investigate the effects of human follicle stimulating hormone (hFSH) on the in vitro culture of caprine preantral follicles. Fragments of goat ovarian cortex were cultured in ?-MEM+ supplemented with 0, 10, or 50 ng/mL hFSH for one or seven days. Small fragments of non-cultured and cultured ovarian tissue were processed for classic histology and transmission electron microscopy. The statistical tests used in this study were Lilliefors, Cochran, and Tukeys (when significance was detected, PROC ANOVA, SAS was used). The results revealed a reduction in the percentage of normal follicles after one or seven days of culture under all treatment conditions. The rates of follicular survival were similar to each other, within each day of culture. The medium containing 10 ng/mL hFSH reduced the percentage of primordial follicles following culture for one and seven days and did not increase the percentage of developing follicles. The follicular diameter of ovarian tissue cultured in ?-MEM+ medium and medium supplemented with 10 ng/mL of hFSH did not change when compared with the control (non-cultured). The ultrastructural analysis confirmed the integrity of follicles cultured for seven days in medium containing 10 ng/mL hFSH. In conclusion, hFSH (10 ng/mL) is capable of promoting the activation of primordial follicles and maintaining the ultrastructural integrity of caprine preantral follicles cultured in vitro for seven days.
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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