Do fibrolytic, proteolytic and amylolytic enzymes influence the in vitro fermentation characteristics of forage? / Enzimas fibrolíticas, amilolíticas e proteolíticas influenciam as características de fermentação in vitro de forragem?
Freiria, Lucien Bissi da; Zervoudakis, Joanis Bissi da; Paula, Nelcino Franciso de; Cabral, Luciano da Silva; Tedeschi, Luis Orlindo; Rosa e Silva, Pedro Ivo José Lopes da; Melo, Alan Carlos Barboza; Possamai, Adriano Jorge.
Semina ciênc. agrar
; 39(3): 1143-1154, 2018. tab, graf
Artículo
en Inglés
| VETINDEX | ID: biblio-1501147
The effects of increasing doses of three exogenous enzymes preparations with fibrolytic activity (FIB- 0, 0.6, 1.2, 1.8, and 2.4 mg mL-¹liquid volume incubated), amylolytic activity (AMZ - 0, 0.05, 0.10,0.15, and 0.20 mg mL-¹liquid volume incubated), and proteolytic activity (PRO - 0, 0.05, 0.10, 0.15, and 0.20 mg mL-¹ liquid volume incubated ) on gas production (GP), kinetic parameters, and fermentation profile of Brachiaria brizantha cv. Marandu were evaluated using the in vitro gas production technique. Ruminal liquid was obtained from two rumen-cannulated Santa Inês sheep maintained on pasture. Accumulated gas production was measured during 96 hours of incubation, measured at 18 different time points. The determined parameters were pH, asymptotic gas production (mL g-¹), rate of gas production (h-¹), lag time (h), organic matter digestibility (OMD, g g-¹ DM), metabolizable energy (ME, MJ kg-¹DM), and neutral detergent fiber digestibility (NDFD, mg g-¹ DM). Increasing the FIB dose linearly increased (P 0.05) asymptotic gas production, but there was quadratic effect (P < 0.05) on the rate of gas production, the lag time, and the GP at the 6-hour and 12-hour time points. The OMD, NDFD and ME were not affected by PRO addition. Thus, fibrolytic, amylolytic and proteolytic enzymes are effective in reducing the lag time and increasing thein vitro gas production from Brachiaria Brizantha cv.[...]
Biblioteca responsable:
BR68.1
Texto completo
Documentos relacionados