Abstract
Purpose:
To evaluate a model for studying the
toxicity in
nervous tissue and
meninges using ultrasound to guide
needle insertion into the
subarachnoid space of
rabbits, with the objective of avoiding
injuries triggered by the
puncture and by intraneural
injection of
solutions.
Methods:
Forty-five
adult female rabbits were divided into 3 groups (G) G1 underwent subarachnoid
puncture, G2 underwent subarachnoid
injection of
saline solution and G3 underwent subarachnoid
injection of 0.5% hyperbaric
bupivacaine. A
needle was inserted into the S1-S2
subarachnoid space guided by ultrasound. The
sensitivity and motility of the
animals were evaluated for 3 days, after which the
animals were sacrificed for removal of lumbar and sacral portions of the
spinal cord for histological examination by
light microscopy and
immunohistochemistry.
Results:
All
animals had motor function and
pain sensitivity on the evaluation period. No
animal had complication during or after the
procedures.
Conclusion:
Ultrasound is a useful and safe
method for the correct identification of the
subarachnoid space of
rabbits.