ABSTRACT Objective
Monocyte chemoattractant protein 1 (MCP-1) has been suggested to be involved in the pathophysiology of
insulin resistance (IR); therefore, variants in the MCP-1
gene may contribute to the development of this
disease. The aim of this study was to analyze the relationship of the -2518 A>G MCP-1 (rs1024611)
gene polymorphism with
insulin resistance in Mexican
children. Subjects and
methods A
cross-sectional study was performed in 174
children, including 117
children without
insulin resistance and 57
children with IR, with an age range of 6-11 years. Levels for
serum insulin and high-
sensitivity C-reactive protein were determined. The -2518 A>G MCP-1 polymorphism was identified by the
polymerase chain reaction-
restriction fragment length polymorphism method.
Insulin resistance was defined as a HOMA-IR in the upper 75th percentile, which was ≥ 2.4 for all
children. Results
Genotype frequencies of the rs1024611 polymorphism for the
insulin-sensitive group were 17% AA, 48% AG and 35% GG, and the frequency of G
allele was 59%, whereas frequencies for the
insulin-resistant group were 12% AA, 37% AG and 51% GG, and the frequency of G
allele was 69%. The
genotype and
allele frequencies between groups did not show significant differences. However, the GG
genotype was the most frequent in
children with IR. The GG
genotype was associated with
insulin resistance (OR = 2.2, P = 0.03) in a
genetic model. Conclusion The -2518 A>G MCP-1
gene polymorphism may be related to the development of
insulin resistance in Mexican
children.