Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales / Frequency of CagA-positive Helicobacter pylori strains in 160 patients subjected to endoscopy
Wormwood, Tracy; Parra, Álvaro; Bresky, Gustavo; Madariaga, Juan A; Häberle, Sergio; Flores, Jacqueline; Bernal, Giuliano.
Rev. méd. Chile
; 146(5): 596-602, mayo 2018. tab, graf
Artículo
en Español
| LILACS | ID: biblio-961436
Background:
Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strains have a higher risk for developing gastric cancer.Aim:
To determine the prevalence of CagA-positive H. pylori strains in fecal samples of patients from the Coquimbo Region of Chile, using a non-invasive, nested-qPCR method. Material andMethods:
We evaluated 160 patients with gastrointestinal symptoms subjected to an upper gastrointestinal endoscopy. DNA was extracted from fecal samples and tested for the presence of H. pylori using nested-qPCR for the ureC gene, and subsequently compared with the results of histology-Giemsa stain from the patients' endoscopic biopsies. When H. pylori was found, the presence of CagA-positive strains was determined via nested-qPCR.Results:
The histology-Giemsa stain was positive for H. pylori infection in 123 patients (76.9%), while the analysis of fecal samples detected H. pylori in 129 patients (80.6%). The sensitivity and specificity of nested-qPCR to detect the bacterium was 96.7 and 73.0% respectively. Among patients with the infection, 25% had CagA-positive strains.Conclusions:
In this sample of patients, there is a low prevalence of CagA-positive H. pylori strains.
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