Artículo
en Inglés
| LILACS, VETINDEX | ID: biblio-976026
Animalpoisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads' secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seqanalysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules.
Methods:
Total RNA from the toadskin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. Furthermore, we performed caseinolytic activity test to assess the presence of serine and metalloproteases in skinsecretion and it was fractionated by fast liquid proteinchromatography using a reverse-phase column. The fractions were partially sequenced by Edman's degradation.
We identified novel toxins and proteins from R. schneideri mucous glands. Besides, this is a pioneer study that presented the in depth characterization of protein molecules richness from this toadsecretion. The results obtained herein showed evidence of novel AMP and enzymes that need to be further explored.(AU)