Molecular analysis of the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children in Rio de Janeiro, Brazil
Araújo, Irene Trigueiros; Heinemann, Marcos Bryan; Mascarenhas, Joana D`Arc P; Assis, Rosane M. Santos; Fialho, Alexandre Madi; Leite, José Paulo G.
J. medical microbiol
; 56: 854-859, 2007. ilus
Artículo
en Inglés
| BVSDIP, FIOCRUZ | ID: dip-2292
Group A rotaviruses are the main cause of acute gastroenteritis in children throughout the world. The two outer capsid proteins, VP4 and VP7, define the P and G genotypes, respectively. Rotaviruses with P[8]G1, P[4]G2, P[8]G3 and P[8]G4 genotypes are predominant in infecting humans and the G9 genotype is emerging in most continents as the fifth most common G type worldwide. The inner capsid protein VP6 is responsible for subgroup (SG) specificities, allowing classification of rotaviruses into SG I, SG 11, SG 1+11 and SG non-l-non-lI. The non-structural protein 4 (NSP4) encoded by segment 10 has a role in viral morphogenesis and five genetic groups have been described, NSP4 genotypes A-E. The aim of this investigation was to characterize the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children. Thirty rotavirus strains were submitted to RT -PCR followed by sequencing and phylogenetic analysis. Among the different G and P genotype combinations, two distinct genetic groups could be recognized for the NSP4 gene. Twenty-eight clustered with NSP4 genotype B. The two P[4]G2 strains fell into NSP4 genotype A and clustered distinctly, with a 100 percent bootstrap value. The strains distinguished within a group were closely related to each other at the nucleotide and...(AU)
Biblioteca responsable:
BR275.1
Ubicación: BR275.1; PCIEC2007
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