Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni
Oliveira, Edward José de; Kanamura, Hermínia Yohko; Takei, kioko; Hirata, Rosario Dominguez Crespo; Valli, Luiz Carlos Pedrosa; Nguyen, Nga Yen; Rodrigues, Izabel de Carvalho; Jesus, Amélia Ribeiro de; Hirata, Mário Hiroyuki.
Trans R Soc Trop Med Hyg
; 102: 360-366, 2008. tab
Artículo
en Inglés
| BVSDIP, FIOCRUZ | ID: dip-2638
pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistsoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (Im-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8 per cent and 94.2 per cent when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in _ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P=0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni
(AU)
Biblioteca responsable:
BR275.1
Ubicación: BR275.1; PCIEC2008
Texto completo
Documentos relacionados