Results on prognostic value of mutations in localized gastrointestinal stromal tumors (GIST) in one single center / Valor pronóstico de las mutaciones en tumores estromales gastrointestinales localizados (GIST): resultados de un solo centro
forty five patients with localized GIST underwent surgery between 1998 and 2010. Thirty six patients were enrolled in a retrospective study. DNA was isolated from 3 to 5 ìm sections of fixed and paraffin-embedded tissue. Exon 9, 11, 13 and 17 of c-kit gene and exon 12 and 18 of PDGFRA were amplified by PCR and sequenced.