Mastitis is one of the most important causes of loss of
cattle production , burdening producers due to the increased
cost of
milk production and decreased herd
productivity . The development of alternative
methods for the
treatment and prevention of
mastitis other than traditional chemical
antibiotic therapy needs to be implemented to meet international
pressures to reduce the use of these
drugs and promote the elimination of multiresistant microbial
strains from the
environment .
Treatment with
probiotic bacteria or
yeast strains offers a possible strategy for the control of
mastitis . The objective of this
work was to isolate, identify, and characterize lactic
bacteria from
milk and the intramammary duct of Gyr, Guzerat, Girolando 1/2, and Holstein
cattle breeds from
Brazil . Samples of 115
cows were taken, a total of 192
bacteria isolates
belonging to 30 species were obtained, and 81 were selected to evaluate their
probiotic potential in
in vitro characterization tests. In general,
bacteria isolated from the
mammary gland have low autoaggregation,
cell surface
hydrophobicity , and co-aggregation with
mastitis etiological
bacteria Staphylococcus aureus and
Escherichia coli . Also, they have
biofilm assembly capacity, inability to produce exopolysaccharides, high
production of H2O2, and strong antagonism against
mastitis pathogens. Ten lactic
bacteria isolates were used in
co-culture with
human MDA-MB-231
breast epithelial cells to assess their adhesion capacity and impairment of the S. aureus invasion. Our results, therefore, contribute to the
future production of new prevention and
treatment tools for
bovine mastitis .(AU)