The studio focused on two cases of IgE-mediated anaphylaxis induced by snailingestion in patients with underlying rhino-conjunctivitis and asthma due to AV. We performed on both patientsskin prick tests ( SPTs) with HA and AV and with a battery of aeroallergen, controlled nasal challenge and specific IgE to HA and AV, ImmunoCAP ISAC®, and a differential pattern of IgE recognition with SDS-PAGEImmunoblotting (SDSI) when these allergens have suffered an O-deglycosylation procedure.
Results:
The patients showed positive results in SPTs, nasal challenges, and serum-specific IgE against HA and AV. In patient 1, the SDSI detected several IgE-binding proteins in AV with a molecular mass of 22, 24, and 44 kDa, whereas a band of 12 kDa was detected in HA. On the other hand, patient 2s serum revealed an IgE-binding zone between 75 and 20 kDa in the AV and a band of 24 kDa in the HA. When glycans were removed, patient 1s serum only revealed the AVs 22 and 24 kDa bands, whereas patient 2s serum did not detect any IgE-reactive protein in the HA.