ARAÚJO, Pedro A. et al. Investigation about the occurrence of transmission cycles of arbovirus in the tropical forest, amazon region. Viruses, v. 11, n. 9, p. 1-10, Sept. 2019.
Artículo
en Inglés
| IED | ID: ied-3926
Because of its ecological characteristics, the Caxiuanã National Forest (FLONA) is a potential area of arbovirus circulation. The present study aimed to investigate the occurrence of arbovirustransmission cycles at FLONA de Caxiuanã. Five field trips were performed to capture mosquitoes and sylvatic vertebrates. For these vertebrates, we attempted viral isolation by cell monolayer inoculation from blood, and hemagglutination inhibition and further seroneutralization assays from sera. For mosquitoes, we performed tests of viral genomedetection. A total of 338 vertebrates were captured, and the greatest representative was birds (251/74.26%). A total of 16,725 mosquitoes were captured, distributed among 56 species. There were no viruses isolated by newbornmouse inoculation. Among birds, antibodies against Ilheus virus were the most prevalent. Catu virus, Caraparu virus, and Mucambo virus were the most prevalent among mammals and reptiles. Fragments of Mucambo virus, Ilheus virus, Bussuquara virus, and Rocio virusgenome were detected in a pool of mosquito samples. These results of the study suggest the occurrence of arbovirustransmission cycles in the FLONA of Caxiuanã. The proximity of humanpopulations with elements, involved in transmission cycles, makes surveillance necessary in this population to avoid dispersion of arboviruses to naïve locations / This work was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), established by notice MCTI/CNPq/FNDCTAção Transversal n68/2013 Programa de Grande Escala da Biosfera-Atmosfera na Amazônia (Processo n 457664/2013-4) to PFV and Instituto Evandro Chagas SVS/MS. PFV is fellow of the CNPq