Selection of lacZ operon fusions in genes of gluconate metabolism in E. coli: Characterization of A gntT::lacZ fusion / Selección de fusiones lacZ de operon en genes del metabolismo del gluconato en E.coli: Caracterización de una fusión gntT::lacZ
Porco, Antonieta; Istúriz, Tomás.
Acta cient. venez
; 42(5): 270-5, 1991. ilus, tab
Artículo
en Inglés
| LILACS | ID: lil-105388
The initial steps involved in the utilization of gluconate by E.coli, its incorporation into the cell and susequent phosphorylation to gluconate 6-phosphate, conform two system that duplicate activities. These system, GntI and GntII, are specified by two sets of genes distinctly regulated and located respectively at the malA-asd (75 min) and fdp-valS (96 min) regions of the bacterial chromosome. The precence of duplicate activities in the metabolism of gluconates of E.coli, has made difficult the study of the expression and participation of the GntI and GntII system. In order to advance in these respec, the phage *placMu53 was used to select operon gntlacZ fusion in a E.coli strain (edd-zwf), (gnd-his), lac. Here we report the study of a gntTlacZ fusion. This transductan allowed to differentiate the inducible expresion of the gntT and gntU genes. its characteristic, in agreement with previous report, support the central role of the gntT gene in this metabolism
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