C-anaphases (Common Variant) are unrelated to colchicine and phytohemagglutinin
Domínguez, María Guadalupe; Rivera, Horacio; Troyo, Rogelio.
Arch. med. res
; 28(1): 25-7, mar. 1997. tab
Artículo
en Inglés
| LILACS | ID: lil-225193
We wvaluated the effect of different concentrations of colchicine (0.0, 0.1, 0.3, 0.5 µg/ml) and phytohemagglutinin (PHA) (0.10, 0.15, 0.20 µg/ml) on the rate of C-anaphases in lymphocyte cultures from five healthy individuals with the common variant of C-anaphases. For each of the 12 possible combinations, two subjects were randomly tested. The frequency of these variant figures was <3 percent; a single culture (out of six with the colchicine concentration of 0.1 µg/ml) lacked C-anaphases. Multiple variance and Student's t tests revealed, as the only significant difference, a decrease with the colchicine concnetration of 0.3 µg/ml compared with the cultures without colchicie (p<0.05), which exhibited the greatest ratio of C-anaphases. The PHA had no influence on the frequency of C-anaphases. We conclude that the common variant of C-anaphases is unrelated to the colchicine and PHA concentrations tested; moreover, our data confirm the occurrence of such a mitotic variant in colchicine-free cultures
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