Detection of intracytoplasmic cytokines by flow cytometry
Santiago, Marta A; Luca, Paula M. De; Bertho, Álvaro L; Azeredo-Coutinho, Rilza B. G; Coutinho, Sérgio G.
Mem. Inst. Oswaldo Cruz
; 95(3): 401-2, May-Jun. 2000.
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| LILACS | ID: lil-258195
Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.
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