Detecçäo de vírus Dengue sorotipos 1 e 2 pela técnica de immunoblotting utilizando proteínas recombinantes / Detection of dengue virus serotypes 1 and 2 by immunoblotting techniques using recombinant proteins
Dengue viroses (DEN-1 to 4) are human pathogens affeeting millions of people In the last two eenturies. Results of a eomparative study between the traditional MAC-ELISAmethod and the Immunoblotting teehniques using reeombinant proteins of DEN-l and DEN-2 to deteet vírus speei- fie class M Immunoglobulins, are reported in this paper. One hundred sera samples were studied. Fifty six samples were positive to dengue by MAC-ELISA and 44 were positive by Immunoblotting teehni- que 36 to DEN-1, 6 to DEN-2 and 2 to DEN-1 and 2 sorotypes. So, the serotype identifieation of the dengue vírus eireulantig in a determined área is possible by using these reeombinant proteins in Immunoblotting teehniques. These data are importante for epidemiologieal surveillanee mainly during the oeeurrenee of an epidemie. (AU)