Alteración en los potenciales de membrana mitocondrial y su relación exponencial con la translocación de fosfatidilserina en la membrana plasmßtica como marcadores en los eventos iniciales de apoptosis: evaluación en diferentes fracciones espermáticas / Changes in mitochondrial membrane potentials and its exponential relation with phosphatidylserine translocation in the plasma membrane as markers in the initial events of apoptosis: evaluation in different spermatic fractions
Villa, Gerardo Barroso; Krivitzky, Samuel Karchmer; Morales, Ernesto Castelazo; Mondragón, Esperanza Carballo; Ambre, Alberto Kably.
Ginecol. obstet. Méx
; 70(4): 182-189, abr. 2002.
Artículo
en Español
| LILACS | ID: lil-331101
OBJECTIVE:
To determine the integrity of the plasmatic membrane through phosphatidylserine (PS) translocation in two spermatic fractions and their correlation with the spermatic mitochondrial membrane potential. MATERIAL ANDMETHODS:
The analysis of both spermatic fractions was carried out through a discontinuous gradient separation with Percoli, in order to obtain two samples with high and low mobility (90-40). Twelve patients were recruited for the initial evaluation of seminal parameters. Mitochondrial membrane integrity was determined using a second antibody (Mitosensor), and was analyzed by fluorescence microscopy, evaluating an average of 200 cells. A 450-490 nm excitation filter was used for this analysis. Cytoplasmatic assessment was carried out by anexine V bonding to PS, in order to determine the initial events of cellular death. Non parameter tests were used in order to determine the differences between mitochondrial potentials and plasmatic membrane processes. Linear correlation tests were used for the anexine V and Mitosensor ratios. RESULTS. Due to the study's design, some differences were observed regarding the displacement parameters and the presence of apoptosis, both, in the plasmatic membrane and in mitochondrial membrane potentials. A positive correlation between both, mitochondrial and cytoplasmic membrane functions was also found. CONCLUSIONS. This is the first study performing a comparative analysis between mitochondrial membrane function and cytoplasmatic PS expression as early cellular death markers. The male infertility population is probably associated with an increase in this kind of apoptosis processes.
Biblioteca responsable:
BR1.1
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