Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
Rezende, Kênnia Rocha; Barros, Sílvia Berlanga de Moraes.
RBCF, Rev. bras. ciênc. farm. (Impr.)
; 40(3): 373-380, jul.-set. 2004. ilus, tab, graf
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| LILACS | ID: lil-420685
The oral dosages formulations from Pothomorphe umbellata (Piperaceae) can only be therapeutically evaluated after establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile in plasma. For that purpose, a unique analytical method validation using HPLC-UV detection was developed for analysis of rat plasma samples. The animals received 10 mg/kg b.w. of 4-NRC i.v.. Analytical conditions were set with C18 column, methanol acetonitrile water (622018) as mobile phase, and a flow rate of 1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The recovery procedure was performed by liquid-liquid extraction showing quantitative extraction (106.4 ± 8.7 por cento)...
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