Effects of plastic stenting in commom bile duct of rats: a quantitative reaction analysis using collagen and elastin morphometry / Efeitos de prótese plástica em hepatocolédoco de ratos: uma análise quantitativa utilizando morfometria do colágeno e elastina
Artifon, Everson Luiz de Almeida; Lopasso, Fabio Pinatel; Moura, Gustavo Brazuna; Fernandes, Fernando Augusto Mardiros Herbella; Sakai, Paulo; Carrilho, Flair José; Caldini, Elia Tamaso Espin Garcia; Silva Junior, Orlando de Castro e.
Acta cir. bras
; 25(2): 153-157, Mar.-Apr. 2010. ilus
Artículo
en Inglés
| LILACS | ID: lil-540491
Purpose:
To evaluate the effects of biliary stenting in rats through analysis of collagen and elastin deposition in the bile ducts.Methods:
Twenty male rats underwent midline laparotomy, duodenotomy and transampullary stenting of the common bile duct with a 22Fr plastic stent. Animals were randomized in 4 groups, with 5 components in each (I) control, (II) biliary stenting for 7 days, (III) biliary stenting for 14 days, and (IV) biliary stenting for 30 days. Sections of the common bile duct were stained using Hematoxylin Eosin; Sirius Red to quantitate the amount of collagen present; and Weigert's Resorcin-fuchsin to quantitate the amount of elastin present.Results:
The percentage of area stained for collagen was 13.4; 21.5; 29.5 and 32.8, for groups I to IV, respectively. The percentage of area stained for elastin fibers was 7.0; 5.2; 4.0 and 2.9, for groups I to IV, respectively. Collagen/Elastin ratio was 2.4; 5.1; 11.0 and 14.4 for groups I to IV, respectively.Conclusions:
The biliary stenting leads to collagen and elastin deposition in the bile ducts; and collagen deposition and collagen/elastin ratio are proportional to the period of stenting.
Biblioteca responsable:
BR1.1
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