Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy / Diagnóstico diferencial no melanoma primário e metastático por espectroscopia FT-Raman
Oliveira, Andrea Fernandes de; Santos, Ivan Dunshee de Abranches Oliveira; Cartaxo, Sidney Bandeira; Bitar, Renata Andrade; Enokihara, Mílvia Maria Simões e Silva; Martinho, Herculano da Silva; Martin, Airton Abrahão; Ferreira, Lydia Masako.
Acta cir. bras
; 25(5): 434-439, Sept.-Oct. 2010. ilus, tab
Artículo
en Inglés
| LILACS | ID: lil-558730
PURPOSE:
To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis.METHODS:
Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm³ and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light NdYAG at 1064 nm was used to excite the inelastic effect.RESULTS:
To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1 percent.CONCLUSION:
FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.
Biblioteca responsable:
BR1.1
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