Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
Ducatti, Diogo R. B; Colodi, Franciely G; Gonçalves, Alan G; Duarte, M. Eugênia R; Noseda, Miguel D.
Rev. bras. farmacogn
; 21(2): 296-304, mar.-abr. 2011. ilus, graf, tab
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| LILACS | ID: lil-590197
Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.
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