Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut
Wang, Chuan Tang; Yu, Shan Lin; Zhang, Shu Wei; Wang, Xiu Zhen; Tang, Yue Yi; Zhang, Jian Cheng; Chen, Dian Xu.
Electron. j. biotechnol
; 13(5): 18-19, Sept. 2010. ilus, tab
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en Inglés
| LILACS | ID: lil-591900
A novel hybrid identification protocol was developed for F01 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.
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