Gelatin,
chitosan and
hyaluronic acid are natural components used to prepare polymeric scaffold in
tissue engineering. The
physical properties of these materials confer an appropriate microenvironment for
cells, which can be used as a
regeneration system for
skin and
cartilage. In this
work, we prepared and characterized a
Gelatin/
Chitosan/
Hyaluronan lyophilized-
polymer.
Physical properties of lyophilized-
polymer changed slightly with moisture, but when
polymer was totally hydrated the
elasticity changed significantly. Thermophysical characterisation indicated that
temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to
protein denaturation. Besides, we used the
polymer as scaffold to prepare a biosynthetic-
skin,
reporting biological behaviour and its mechanical properties.