Screening of
peanut seeds resulting from 0.39 percent
sodium azide treatment with NIRS
calibration equation for bulk
seed samples identified a
plant with more than 60 percent
oleate.
Oleate content in individual
seeds of the
plant, as predicted by NIRS
calibration equation for intact single
peanut seeds, ranged from 50.05 percent ~ 68.69 percent. Three
seeds with >60 percent
oleate thus identified were further confirmed by
gas chromatography. Multiple
sequence alignments of the FAD2B
gene from Huayu 22 (wild type) and
peanut seeds with elevated
oleate (mutant type) revealed a C281T transition in the
coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the
enzyme activity.