Aumento del daño en el ADN espermático en varones mayores de 40 años / Association of age with sperm DNA fragmentation
Horta, Fabrizzio; Madariaga, Marcia; García, Alejandra; Hartel, Steffen; Smith, Rosita.
Rev. méd. Chile
; 139(3): 306-312, mar. 2011. ilus
Artículo
en Español
| LILACS | ID: lil-597618
Background:
There is an association between aging ana an increased number ofsperms with alterations in nuclear DNA.Aim:
To study the association between age and fragmentation of sperm DNA. Material andMethods:
Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TÚNEL (terminal deoxynucleotidyl transferase-mediated2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersión test) assays.Results:
Compared with theiryounger counterparts, patients aged more than 40years had a higher proportion ofsperms with DNA fragmentation by TÚNEL (20 ±1.3 and24 ± 1.9 percent respectively, p < 0.05) and SCD (22 ± 1.4 and26 ± 1.6 percent respectively, p < 0.05). The results ofboth assays had a correlation coefficientofO.8. No differences between groups were observed for other seminal parameters.Conclusions:
Sperm DNA fragmentation increases with age in males.
Biblioteca responsable:
BR1.1
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