Heat identification by 17beta-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay
Domènech, Anna; Pich, Sara; Arís, Anna; Plasencia, Carmen; Bach, Alex; Serrano, Alicia.
Electron. j. biotechnol
; 14(4): 10-10, July 2011. ilus, tab
Artículo
en Inglés
| LILACS | ID: lil-640505
Background:
There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17beta-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17beta-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk.Results:
Milk sample treatments for progesterone and 17beta-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17beta-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17beta-estradiol pre-ovulation peak.Conclusions:
17beta-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination.
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