The P1.HTR
cell line includes highly transfectable
cells derived from P815
mastocytoma cells originating from
mouse breast tissue. Despite its widespread use in immunogenic studies, no data are available about the
behavior of P1.HTR
cells in the
chick embryo chorioallantoic membrane model. The objective of the present investigation was to study the effects of P1.HTR
cells implanted on the
chorioallantoic membrane of
chick embryos. We inoculated P1.HTR
cells into the previously prepared
chick embryo chorioallantoic membrane and observed the early and late effects of these
cells by stereomicroscopy, histochemistry and
immunohistochemistry. A highly angiotropic and angiogenic effect occurred early after inoculation and a tumorigenic potential with the development of
mastocytoma keeping well
mast cells immunophenotype was detected later during the development. The P1.HTR
mastocytoma cell line is a good tool for the development of the
chick embryo chorioallantoic membrane mastocytoma model and also for other studies concerning the involvement of
blood vessels. The
chick embryo chorioallantoic membrane model of
mastocytoma retains the
mast cell immunophenotype under experimental conditions and could be used as an experimental tool for in vivo preliminary testing of antitumor and antivascular
drugs.