Bacillus sphaericus AND 303, a Cr(VI)-resistant and reducing bacterium reported from serpentine outcrops of Andaman was evaluated for Cr(VI) reduction using immobilized cells under batch culture. Screening of inert matrices for entrapment of whole cells indicated that polyvinyl alchohol-alginate was the most effective one reducing 87.5% of 20 µM Cr(VI) in 24 h. The rate of chromate reduction was dependent on initial Cr(VI) and biomass concentrations. The PVA cell beads were recycled three times without cellleakage and disintegration. The reduction efficiency was improved in the presence of glucose and glycerol as electrondonors leading to complete reduction. However, the presence of additional metalions was inhibitory to Cr(VI) reduction. It could be emphasized that PVA-alginateimmobilized cells of B. sphaericus AND 303 could be used as a continuous bioprocess in treating Cr(VI) contaminated effluents.