Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times / Modulação dos receptores de hormônio tireoidiano, TRα e TRβ, utilizando diferentes doses de triiodotironina (T3) em diferentes tempos
Oliveira, Miriane de; Luvizotto, Renata de Azevedo Melo; Olimpio, Regiane Marques Castro; Sibio, Maria Teresa de; Silva, Carolina Biz Rodrigues; Conde, Sandro José; Padovani, Carlos Roberto; Nogueira, Célia Regina.
Arq. bras. endocrinol. metab
; 57(5): 368-374, jul. 2013. ilus, graf, tab
Artículo
en Inglés
| LILACS | ID: lil-680624
OBJECTIVE:
To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times. MATERIALS ANDMETHODS:
3T3-L1 adipocytes were incubated with T3 (physiological dose F; supraphysiological doses SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction.RESULTS:
F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group.CONCLUSIONS:
T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
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BR1.1
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