Standardization of pulmonary ventilation technique using volume-controlled ventilators in rats with congenital diaphragmatic hernia / Padronização da técnica de ventilação pulmonar utilizando ventiladores com volume controlado em ratos com hérnia diafragmática congênita
Gallindo, Rodrigo Melo; Gonçalves, Frances Lilian Lanhellas; Figueira, Rebeca Lopes; Simões, Ana Leda Bertoncini; Sbragia, Lourenço.
Rev. Col. Bras. Cir
; 41(3): 181-187, May-Jun/2014. tab, graf
Artículo
en Inglés
| LILACS | ID: lil-719488
OBJECTIVE:
To standardize a technique for ventilating rat fetuses with Congenital Diaphragmatic Hernia (CDH) using a volume-controlled ventilator.METHODS:
Pregnant rats were divided into the following groups a) control (C); b) exposed to nitrofen with CDH (CDH); and c) exposed to nitrofen without CDH (N-). Fetuses of the three groups were randomly divided into the subgroups ventilated (V) and non-ventilated (N-V). Fetuses were collected on day 21.5 of gestation, weighed and ventilated for 30 minutes using a volume-controlled ventilator. Then the lungs were collected for histological study. We evaluated body weight (BW), total lung weight (TLW), left lung weight (LLW), ratios TLW / BW and LLW / BW, morphological histology of the airways and causes of failures of ventilation.RESULTS:
BW, TLW, LLW, TLW / BW and LLW / BW were higher in C compared with N- (p <0.05) and CDH (p <0.05), but no differences were found between the subgroups V and N-V (p> 0.05). The morphology of the pulmonary airways showed hypoplasia in groups N- and CDH, with no difference between V and N-V (p <0.05). The C and N- groups could be successfully ventilated using a tidal volume of 75 ìl, but the failure of ventilation in the CDH group decreased only when ventilated with 50 ìl.CONCLUSION:
Volume ventilation is possible in rats with CDH for a short period and does not alter fetal or lung morphology. .
Biblioteca responsable:
BR1.1
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