Background: It is believed that
Human Papillomavirus (HPV) and
Human Immunodeficiency Virus coinfection contributes to increase the
risk for cervical intraepithelial
injuries . Several factors may contribute to
cervical cancer (CC) development, including genetic variants such as TP53 and MDM2
gene polymorphisms . Materials and
methods: A hundred
HIV -infected
women were examined for HPV
detection and its
genotypes , as well as the frequencies of the
SNPs Arg72Pro and SNP309 and their
associations with CC
risk factors .
Nested Polymerase Chain Reaction (
nPCR ) was used for HPV
detection and
PCR -
RFLP for TP53 and MDM2 SNP309 genotyping.
Results: HPV
DNA was detected in 68% of samples. A higher frequency of low-
risk HPV
genotypes (66.7%) was observed when compared to high-
risk genotypes (33.3%). Nine different HPV
genotypes were identified, with the highest
prevalence of
HPV-6 , followed by HPV-16 and 31. p53 Arg72Arg and SNP309 TG
genotype were the most prevalent. HPV genotyping was performed by sequencing.
Conclusion: The data obtained suggest that
HIV -infected
women are more susceptible to be infected by low-
risk HPV (LR-HPV)
genotypes than by high-
risk (HR-HPV), and Pro72Pro of
TP53 gene and TG of MDM2 SNP309
genotypes apparently seem to be
protective factors among
HIV -infected
women for HPV acquisition and HR-
HPV infection , respectively, in a sample of Southern Brazilian
woman .
Future investigations in larger
populations are necessary to better understand the potential
roles of these
SNPs and the
behavior of non-oncogenic HPV
genotypes in
HIV -mediated
immunosuppression cases. .