Antibacterial properties of silver nanoparticles synthesized by marine Ochrobactrum sp.
Thomas, Roshmi; Janardhanan, Anju; Varghese, Rintu T.; Soniya, E.V.; Mathew, Jyothis; Radhakrishnan, E.K..
Braz. j. microbiol
; 45(4): 1221-1227, Oct.-Dec. 2014. ilus, graf, tab
Artículo
en Inglés
| LILACS | ID: lil-741271
Metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. Biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. In the present study, an eco favorable method for the biosynthesis of silver nanoparticles using marine bacterial isolate has been attempted. Very interestingly, molecular identification proved it as a strain of Ochrobactrum anhtropi. In addition, the isolate was found to have the potential to form silver nanoparticles intracellularly at room temperature within 24 h. The biosynthesized silver nanoparticles were characterized by UV-Vis spectroscopy, transmission electron microscope (TEM) and scanning electron microscope (SEM). The UV-visible spectrum of the aqueous medium containing silver nanoparticles showed a peak at 450 nm corresponding to the plasmon absorbance of silver nanoparticles. The SEM and TEM micrographs revealed that the synthesized silver nanoparticles were spherical in shape with a size range from 38 nm - 85 nm. The silver nanoparticles synthesized by the isolate were also used to explore its antibacterial potential against pathogens like Salmonella Typhi, Salmonella Paratyphi, Vibrio cholerae and Staphylococcus aureus.
Asunto(s)
Antibacterianos/farmacología Nanopartículas/metabolismo Ochrobactrum/metabolismo Plata/farmacología Antibacterianos/metabolismo Organismos Acuáticos/clasificación Organismos Acuáticos/genética Organismos Acuáticos/aislamiento & purificación Organismos Acuáticos/metabolismo Análisis por Conglomerados ADN Bacteriano/química ADN Bacteriano/genética ADN Ribosómico/química ADN Ribosómico/genética Bacterias Gramnegativas/efectos de los fármacos Microscopía Electrónica Nanopartículas/química Nanopartículas/ultraestructura Ochrobactrum/clasificación Ochrobactrum/genética Ochrobactrum/aislamiento & purificación Filogenia /genética Análisis de Secuencia de ADN Análisis Espectral Plata/metabolismo Staphylococcus aureus/efectos de los fármacos Temperatura Factores de Tiempo
Biblioteca responsable:
BR1.1
powered by iAHx-2.18-89
Biblioteca Virtual en Salud