Determination of lytic enzyme activities of indigenous Trichoderma isolates from Pakistan
Asad, Saeed Ahmad; Tabassum, Ayesha; Hameed, Abdul; Hassan, Fayyaz ul; Afzal, Aftab; Khan, Sabaz Ali; Ahmed, Rafiq; Shahzad, Muhammad.
Braz. j. microbiol
; 46(4): 1053-1064, Oct.-Dec. 2015. tab, graf
Artículo
en Inglés
| LILACS | ID: lil-769641
Abstract This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sugar reduction by dinitrosalicylic acid (DNS) method using suitable substrates. The antagonists were cultured in minimal salt medium with the following modifications medium A (1 g of glucose), medium B (0.5 g of glucose + 0.5 g of deactivated R. solani mycelia), medium C (1.0 g of deactivated respective antagonist mycelium) and medium D (1 g of deactivated R. solani mycelia). T asperellum showed presence of higher amounts of chitinases, β-1, 3-glucanases and xylanases in extracellular protein extracts from medium D as compared to medium A. While, the higher activities of glucosidases and endoglucanses were shown in medium D extracts by T. harzianum. β-glucosidase activities were lower compared with other enzymes; however, activities of the extracts of medium D were significantly different. T. asperellum exhibited maximum inhibition (97.7%). On the other hand, Trichoderma sp. did not show any effect on mycelia growth of R. solani on crude extract.
Asunto(s)
Quitinasas/análisis Quitinasas/química Quitinasas/enzimología Quitinasas/crecimiento & desarrollo Quitinasas/metabolismo /análisis /química /enzimología /crecimiento & desarrollo /metabolismo Proteínas Fúngicas/análisis Proteínas Fúngicas/química Proteínas Fúngicas/enzimología Proteínas Fúngicas/crecimiento & desarrollo Proteínas Fúngicas/metabolismo Glicósido Hidrolasas/análisis Glicósido Hidrolasas/química Glicósido Hidrolasas/enzimología Glicósido Hidrolasas/crecimiento & desarrollo Glicósido Hidrolasas/metabolismo Micelio/análisis Micelio/química Micelio/enzimología Micelio/crecimiento & desarrollo Micelio/metabolismo Pakistán/análisis Pakistán/química Pakistán/enzimología Pakistán/crecimiento & desarrollo Pakistán/metabolismo Trichoderma/análisis Trichoderma/química Trichoderma/enzimología Trichoderma/crecimiento & desarrollo Trichoderma/metabolismo
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