Clinical characteristics and frequency of TLR4 polymorphisms in Brazilian patients with ankylosing spondylitis / Características clínicas e frequência de polimorfismos em TLR4 em pacientes brasileiros com espondilite anquilosante
Machado, Natalia Pereira; Nogueira, Eliana; Oseki, Karen; Ebbing, Pâmela Carolina Cruz; Origassa, Clarice Silvia Taemi; Mohovic, Tatiane; Câmara, Niels Olsen Saraiva; Pinheiro, Marcelo de Medeiros.
Rev. bras. reumatol
; 56(5): 432-440, Sept.-Oct. 2016. tab
Artículo
en Inglés
| LILACS | ID: lil-798096
ABSTRACT
Objectives:
Innate immunity is involved in the physiopathology of ankylosing spondylitis (AS), with the participation of Gram-negative bacteria, modulation of human leukocyte antigen (HLA) B27 and the involvement of pattern recognition receptors, such as Toll-like receptors (TLRs). The aim of this study was to investigate the clinical characteristics and frequency of TLR4 polymorphisms (Asp299Gly and Thr 399Ile) in a cohort of Brazilian patients with AS.Methods:
A cross-sectional study was carried out involving 200 patients with a diagnosis of AS and a healthy control group of 200 individuals. Disease activity, severity and functional capacity were measured. The study of TLR4 polymorphisms was performed using the restriction fragment length polymorphism method. HLA-B27 was analyzed by conventional polymerase chain reaction. The IBM SPSS Statistics 20 program was used for the statistical analysis, with p-values less than 0.05 considered significant.Results:
Mean age and disease duration were 43.1 ± 12.7 and 16.6 ± 9.2 years, respectively. The sample was predominantly male (71%) and non-Caucasian (52%). A total of 66% of the group of patients were positive for HLA-B27. The sample of patients was characterized by moderate functional impairment and a high degree of disease activity. No significant association was found between the two TLR4 polymorphisms and susceptibility to AS.Conclusions:
TLR4 polymorphisms 399 and 299 were not more frequent in patients with AS in comparison to the health controls and none of the clinical variables were associated with these polymorphisms.
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BR1.1
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